Stem Cell Procedures
Magellan Stem Cells operate a clean room laboratory facility in Melbourne, Australia. Isolation and expansion of a `pure’ stem population are undertaken using internationally accepted techniques. All cells are characterized by Flow Cytometry (FACS) using standards set by the International Society for Cellular Therapy. At critical processing points the cell sample undergoes sterility testing using an independent pathology provider. Stem cells are stored for future use in sterile cyrovials using validated methods.
Isolation and Expansion
These autologous treatments require that a small amount of adipose tissue (‘fat’) is first sourced/removed from the patient in order to ‘obtain cells’. This fat/tissue is normally obtained via a small lipo-suction procedure. Following the lipo-suction, the cells in the adipose tissue (stromal vascular fraction – ‘SVF’) are first isolated from the adipose/fat using a GMP grade reagent. This process typically takes 3-4 hours. The isolated stem cells are then separated/purified from all of the other cells in the SVF. This process typically takes 6-10 days. (PS - only about 5% - 10% of the SVF are ‘isolated’ stem cells.).
The isolated stem cells are then expanded (increased in number) such as to provide the required number of stem cells for the treatment(s). The cells are isolated, purified and expanded using strict laboratory protocols that have been designed to ensure optimal processing and adherence to strict standards re sterility etc. All cells are independently tested for bacterial and fungal infections prior to release for the treatment of patients.